ODDIY SACHRATQI (CICHORIUM INTYBUS L) OʼSIMLIK QISMLARIDAN ENDOFIT BAKTERIYALARNING SOF KULTURALARINI AJRATISH USULLARI
Keywords:
Cichorium intybus, MALDI TOF mass-spektrometriya, Bacillus thuringiensis, Bacillus cereus, Bacillus pumiluslar, dominant shtamAbstract
Oddiy Sachratqi (Cichorium intybus L) oʼsimlik qismlaridan endofit bakteriyalarning sof kulturalarini ajratish uslublarini tahlil qilishda dastlab o`simlik namunalari yig`ildi. O`simlikning kerakli qismlari tanlab olinib, strillandi. Ushbu namunalar asosida 18 ta izolyatlar olindi va Chapek va kartoshka glyukozali ozuqa muhitiga ekildi. Olingan izolyatlarni MALDI TOF mass-spektrometriya usulida identifikatsiya qilish natijasida, Bacillus thuringiensis, Bacillus cereus, Bacillus pumiluslar borligi aniqlandi. 18 ta izolyatdan 9 tasida aniqlanib, Bacillus thuringiensis shtammi dominantlik qilganligi sababli, ushbu shtammning fitopatogen mikroorganizmlarga nisbatan antagonistik xususiyatini aniqlash ustida keyingi ishlar davom ettirildi.
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Oʻsimlik oʻsishi va rivojlanishida foydali mikroorganizmlarning ahamiyati
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